Three other instances of ovarian clear-cell carcinoma also appear to be characterized by loss of heterozygosity, based on the frequency of mutant alleles and wild-type alleles and subsequent loss of BAF250a expression. However, nearly all cancers with somatic ARID1A mutations and loss of BAF250a expression may actually have a wild-type allele present. Data from exon resequencing and RNA sequencing show excellent agreement between your fraction of mutant and wild-type alleles at both DNA and RNA amounts , suggesting that epigenetic silencing is not a significant factor. Post-transcriptional or post-translational regulation or dominant unwanted effects of the mutations are possible, albeit untested, explanations for having less proteins expression in these heterozygous instances. The presence of BAF250a immunoreactivity in 15 samples positive for an ARID1A mutation may indicate that haploinsufficiency is pathogenic, as has been reported in mice.32 Alternatively, immunohistochemical detection of a truncated but nonfunctional BAF250a proteins may account for the immunostaining results.USA Today: Seniors' Use Of Potent Meds Via Medicare Staggering The number of senior citizens getting narcotic painkillers and anti-anxiety medicines under Medicare's prescription drug program sharply is climbing, and the ones older patients are being put on the medications for longer periods of time, a USA TODAY examination of federal data shows. From 2007-2012, the number of patients 65 and old getting Medicare prescriptions for powerful opioid pain medicines rose more than thirty % to upward of 8.5 million beneficiaries, the data show . Related StoriesUse of observation remains may lead to economic burden for a few Medicare patientsMembers signed up for Medicare Advantage satisfied with their plans, research findsCost of radiation therapy varies widely among Medicare sufferers with cancerNPR: Switching To Newer Insulin For Type 2 Diabetes Comes At A Cost Many people who have diabetes possess switched to newer types of insulin known as analogs, because the disease can be created by them simpler to manage.